Tuesday, February 10, 2015

The Best Kit For Solubility Screening

By Beryl Dalton


Protein aggregation affects their availability for certain functions. This calls for solubility screening to establish the formulations or conditions that will protect your proteins. The choice of kit will determine the comprehensiveness or intensity of the results you obtain. The protein samples are solubilized during testing depending on expectations.

The kit allows you to use salt, additives and PH to detect quick aggregation capabilities or properties. This kit allows testing based on several conditions in parallel and label free forms. You are not required to use centrifuge during the testing. Simple mixing and spinning produces the desired results. This means that you can obtain desired results in different circumstances.

Screening kits allow you to rescue protein samples that are reversibly aggregated. The kit also performs incredibly well in protecting proteins from aggregating when under stress. This means that they will be available in the form desired. The trends used on such kits are easily found on the dash board. Your results are easily related to what is conventionally known.

Protein aggregation is an undesirable process that is attributed to a number of conditions. These conditions include vigorous stirring, elevated temperatures, addition of molecular binding partners or ligands and prolonged storage of protein. It is possible to avoid aggregation by making the right choices on stabilizing additives, PH and salt.

The testing kit comes with buffers of PH that ranges from three to ten. You will also get solubility enhancers like polyols, amino acids, salts, reducing agents and sugars. These enhancers give precise results on the conditions in which your proteins will aggregate or de-aggregate.

A single label free experiment can be used to explain over ninety formulations. The control tests are six with yes and no possibilities. A basic test is where soluble proteins go through the filters while insoluble proteins do not. This is a clear indication of the properties of these proteins that can be used to draw inferences and make conclusions.

Protein solubilization tests give the conditions under which your proteins will not aggregate. From such tests, it is observed that filtration processes yield proteins when they are in soluble form. They are available in aggregated form if the test does not show any traces of proteins.

Screening results will point at the conditions in which the proteins exist. The results allow you to identify well behaved proteins that will serve your intended purpose. This confirmation is necessary if expected results are to be obtained when such proteins are used in large scale.

You stand to enjoy numerous benefits using this kit than other methods available in the market. It produces clear results that are easy to interpret based on the conditions you are testing. It also is fast which allows you to get results within an hour. This places it at a better position compared to the trial and error methods used in other quotas.

The amount of protein you will require is substantially reduced. This allows it to be evenly matched to the target detection assay. This method is also convenient since it provides a lot of information in one test. The kit also allows you to use your assay of choice without being restricted to certain formulations.




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